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Suitability of purified RNA for downstream processes. Total RNA from eight samples purified using the automated HTP RNA extraction procedure was analysed for IPP2 expression by reverse transcription and qPCR. No expression was detected in RT- (reverse transcriptase minus) samples (data not shown) or water. Data are presented as the mean ± SD of triplicate qPCR experiments and demonstrate the consistent high quality of the purified RNA.
