Table 1.
Human liver protein secretion from transplanted human iPSC-derived hepatic cells. Human liver protein secretion was measured using ELISA for each protein in serum or plasma obtained from mice that had been transplanted with hepatic cells (2 × 106 cells per mouse) at different differentiation stages derived from human ESCs or iPSCs, or transplanted with primary human hepatocytes (PHs). Both undifferentiated (day 0) iPSCs and PH cells were used as controls. There were no marked differences in albumin secretion for hepatic cells derived from human iPSCs of different origins (A), although the concentrations of albumin secreted by these hepatic cells were higher than for undifferentiated iPSCs. Similar results were observed for the secretion of other liver proteins (transferrin, AAT, and fibrinogen) (B) (results of human endoderm–derived iPSCs are shown). DE, definitive endoderm; HP, hepatic progenitors; MH, mature hepatocyte-like cells derived from human iPSCs; PSCs, pluripotent stem cells; iPSend, endoderm-derived human iPSCs; iPSmes, mesoderm-derived human iPSCs; iPSect, ectoderm-derived iPSCs; ND, not detected.
| Day 0 (iPSCs) | Day 5 (DE) | Day 10 (HP) | Day 20 (MH) | PHs | |
|---|---|---|---|---|---|
| A. Human albumin secretion from distinct origin human iPSC- and ESC-derived hepatic cells in host serum (n = 3 to 10, means ± SEM) | |||||
| Human PSCs (ng/ml) | |||||
| H1, H9 (ESCs) | 1.9 ± 0.5 | 50 ± 12 | 35 ± 10 | 38 ± 11 | |
| iH6-14 (iPSend) | 1.5 ± 0.5 | 46 ± 13 | 37 ± 11 | 40 ± 9 | |
| iM2-9, iLC1-2 (iPSmes) | 2.1 ± 0.8 | 48 ± 14 | 34 ± 9 | 38 ± 12 | 40 ± 8 |
| iK1-3 (iPSect) | 1.8 ± 0.5 | 45 ± 11 | 32 ± 12 | 39 ± 10 | |
| B. Human liver protein secretion from human endoderm iPSC-derived hepatic cells in host serum/plasma (n = 4 to 10, means ± SEM) | |||||
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| |||||
| Transferrin (ng/ml) | 9 ± 0.8 | 101 ± 18 | 62 ± 13 | 86 ± 15 | 58 ± 11 |
| AAT (ng/ml) | 1.1 ± 0.5 | 8.1 ± 1.4 | 7.8 ± 1.1 | 7.9 ± 1.2 | 8.2 ± 1.3 |
| Fibrinogen (μg/ml) | ND | 1.1 ± 0.2 | 0.9 ± 0.1 | 0.9 ± 0.1 | 0.9 ± 0.1 |