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. 2012 Mar 1;26(5):433–438. doi: 10.1101/gad.179416.111

Figure 1.

Figure 1.

Identification of mH2A1.2 chromatin-free interacting factors. (A) Fluorescence microscopy of HEK293 cells stably expressing GFP-H2A and mH2A1.2-GFP. Arrowhead indicates Xi. (B) Procedure used to isolate chromatin-free H2A- and mH2A1.2-interacting factors. (C) αGFP immunoblot confirms expression and immunoprecipitation of histones in stable cell lines. (D) Immunoblots of Imp9, Npm, and NAP1 association with GFP-H2A or mH2A-GFP isoforms. Arrows on all αGFP blots indicate GFP-H2A (bottom) and mH2A-GFP (top), and asterisks indicate degradation products.