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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Bone Miner Res. 2011 Sep;26(9):2151–2160. doi: 10.1002/jbmr.425

Figure 1.

Figure 1

Study design and effect of BtxA injection on muscle function and mass. A. Diagrammatic representation of the study design. A total of 40 mice were used, 20 of each genotype. Twenty mice were sacrificed 3 weeks after intramuscular injection of botulinum toxin A (BtxA) or saline (5 per treatment for each genotype); and 20 were followed up to 19 weeks (5 per treatment for each genotype). The mice followed for 19 weeks had in vivo μCT scans every 4 weeks. B. Changes in muscle function assessed by Digital Abduction Score (DAS) after BtxA injection. Muscle paralysis (DAS=4) and recovery were similar in wild type (WT) and conditional knockout (cKO). C. Change in muscle area measured by μCT in WT and cKO mice followed for 19 weeks. There was a significant effect of treatment on muscle area by 2-way ANOVA (p<0.05); p < 0.05. a: p < 0.05 vs cKO Saline; b: p < 0.05 vs WT Saline.