Effect of botulinum toxin A (BtxA) on cortical bone in wild type (WT) and
Gja1 conditional knockout (cKO). A. Difference
in Marrow Area (Ma.Ar.) between injected (BtxA or saline) and non-injected
control limbs, expressed as a percentage of baseline. There was significant
expansion of the marrow cavity for WT mice beginning 3 weeks post BtxA injection
and persisting through the 19 weeks of the study. There was a significant effect
of genotype and treatment by 2-way ANOVA (p < 0.05). B.
Difference in Cortical Area (Ct.Ar) between injected (BtxA or saline) and
non-injected control limbs, expressed as a percentage of baseline. There was a
significant reduction in relative cortical area over time for the WT BtxA mice.
There was a significant genotype effect in the BtxA group (p<0.05) but not in
the Saline mice by 2-way ANOVA (p>0.05). No significant changes in Ct.Ar were
observed for the cKO BtxA mice over the 19 weeks of the study. C.
Micro-CT generated 3-D reconstruction of diaphyses of WT and cKO taken at
baseline, 3 weeks and 19 weeks for each genotype. a: p < 0.05 vs cKO Saline,
b: p < 0.05 vs WT Saline, c: p < 0.05 vs cKO BtxA, d: p < 0.05 vs WT
Baseline.