. Author manuscript; available in PMC: 2012 Mar 16.

Published in final edited form as: Int J Mass Spectrom. 2009 Oct 15;287(1-3):96–104. doi: 10.1016/j.ijms.2008.10.017

Table 2.

ΔG_H₂O and m_GdHCl values obtained for staphylococcal nucleases at room temperature ~25°C using the PEPS HX ESI-MS method compared to fluorescence values [5, 6,36]. Units of ΔG_H₂O and mGdHCl units are kcal mol⁻ and kcal mol⁻¹ M⁻¹ respectively. Estimated average uncertainty for the ΔG_H₂O is ~0.3 kcal/mol and for m_GdHCl it is 0.1 kcal mol⁻¹ M⁻¹.

Staphylococcal Nuclease	ΔG_H₂O (MS)	ΔG_H₂O (Fluoresence)	m_GdHCl(MS)	m_GdHCl(Fluoresence)
Wild Type	4.7	5.0^*	6.3	6.5^*
23I/25I/66L/72L	2.4	2.7	6.5	6.9
117G/124L/128A/41I/59A/21K/21N	11.1	11.0	5.4	5.3

measured at 25°C