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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Science. 2011 Nov 24;334(6063):1706–1710. doi: 10.1126/science.1211222

Fig. 2.

Fig. 2

Characterization of Lmnb1−/−Lmnb2−/− ESCs. (A and B) Protein immunoblotting analyses of lamin-B1, -B2, and -B3 (B3 shares the same C terminus as B2). Wild-type testes lysates were used as positive controls for lamin-B3. β-Actin was used as a loading control. wt, LB1−/−, LB2−/−, and DKO represent Lmnb1+/+Lmnb2+/+, Lmnb1−/−Lmnb2+/+, Lmnb1+/+Lmnb2−/−, and Lmnb1−/−Lmnb2−/−, respectively. (C) Immunofluorescence staining of ESCs with antibodies to lamin-B1 (LB1), lamin-B2 (LB2), or emerin. DNA was counterstained with Hoechst dye. Scale bars, 10 µm. (D) Electron micrographs of ESCs. N, nucleus; C, cytoplasm; NUP, nuclear pore; INM, inner nuclear membrane; ONM, outer nuclear membrane. Scale bars, 1 µm (whole-cell views) and 0.1 µm (enlarged views).