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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Nature. 2005 Nov 6;439(7074):358–362. doi: 10.1038/nature04304

Figure 4. PD0325901 completely suppresses the growth of BRAF(V600E) mutant xenografts.

Figure 4

a, PD0325901 suppressed the growth of SKMEL28 (BRAF(V600E)) xenografts at both the 5 and 25 mg kg−1 dose levels. b, c, In contrast, 5 mg kg−1 PD0325901 only delayed the growth of SKMEL103 (RAS(Q61R)) and SKMEL31 (RAS/RAF-WT) xenografts, with complete growth suppression requiring the higher dose. d, BT-474 xenografts (RAS/RAF-WT) were refractory to MEK inhibition (n = 10 mice per group). e, f, PD0325901 reduced p-ERK levels in both SKMEL28 and BT-474 xenograft tumours but caused downregulation of D-cyclins, induction of p27 and hypophosphorylation of RB, and a decline in the proliferative index only in the SKMEL28 xenografts. Tumour lysates were derived from mice euthanized 8 h after the final treatment. All error bars indicate standard error.