Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Curr Protoc Pharmacol. 2012 Mar;CHAPTER:Unit2.8. doi: 10.1002/0471141755.ph0208s56

Figure 2. Example RGS protein/Gα FRET experimental setup.

Figure 2

A) Each reaction mixture consists of 150 µL 3X RGS protein solution, 150 µL 3X Gαi1, and compound or DMSO to the desired test concentration. The total reaction volume is 450 µL. B) The reaction mixtures, in both AMF and GDP buffers, are distributed in triplicate across a 96-well plate. The shading gradient represents varying concentrations of test compound.