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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Curr Protoc Pharmacol. 2012 Mar;CHAPTER:Unit2.8. doi: 10.1002/0471141755.ph0208s56

Figure 5. RGS4 is allosterically modulated by phospholipids in single turnover hydrolysis assays.

Figure 5

Phosphatidyl inositol triphosphate (PIP3) modulates RGS4 activity though an allosteric “B” site, described previously (Ishii and Kurachi, 2004; Tu and Wilkie, 2004). In single turnover assays, inclusion of phospholipid vesicles containing PIP3, but not phosphatidyl choline (PC) only, inhibits RGS4 GAP activity. Similar reversal of RGS4-mediated GTP hydrolysis acceleration would be expected for an inhibitory compound.