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. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: J Mol Biol. 2012 Jan 28;417(1-2):112–128. doi: 10.1016/j.jmb.2012.01.011

Figure 6.

Figure 6

The comparative best fits of stopped flow and titration data at low [Ca2+] (grey solid lines) by the Hill model for the two sets of reverse constants: (i) High [Ca2+] reverse rate constants k− 1 =500 s−1, Y2= 0.208 denoted as dark gray dotted lines and (ii) Chen’s reverse rate constants at low [Ca2+] k−1 = 3 s−1 and Y2 =0.0703 denoted as black dashed lines. A) For the excess F-actin (2.5 μM pyrene actin) vs 0.25 μM S1 after mixing and for the excess S1 (5 μM) vs. 0.5 μM pyrene actin both at pCa=8.9. B) For titrations at pCa=8.9.