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. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: J Mol Biol. 2012 Jan 28;417(1-2):112–128. doi: 10.1016/j.jmb.2012.01.011

Figure 9.

Figure 9

Stopped flow transients of mixing excess of S1 with pyrene actin to quantitatively demonstrate the calcium independent slowing of S1 binding at higher occupancy of actin sites by bound S1. A) The excess S1 stopped flow experiment: 5 μM S1 was quickly mixed with 50 nM pyrene actin in presence of 20 nM TmTn. B) Excess S1 stopped flow experiment repeated with actin preloaded with S1 at 5:4 ratio of A:S1. 50 nM pyrene actin was equilibrated with 40 nM S1 in presence of 20 nM TmTn and then rapidly mixed with 5 μM S1. The same pCa values and color codes were used as in A.