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. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: J Mol Biol. 2012 Jan 28;417(1-2):112–128. doi: 10.1016/j.jmb.2012.01.011

Table 4.

Estimated kinetic rate constants of the McKillop-Geeves stochastic model from the best fits of the family of stopped flow transients when [S1] is in excess to [actin].

Calcium Concentration
pCa 4.6 5.4 5.8 6.2 6.4 6.8 7 8.9
kB 6000 2233.50 336.30 62.00 32.50 19.08 16.70 12.66
k−B 100 100 100 100 100 100 100 100
kT 529.5 563.7 560.1 366.6 319.5 233.1 217.8 167.1
k−T 3000 3000 3000 3000 3000 3000 3000 3000
k1 2.2 2.2 2.2 2.2 2.2 2.2 2.2 2.2
k−1 10 10 10 10 10 10 10 10
k2 1000 1000 1000 1000 1000 1000 1000 1000
k−2 5 5 5 5 5 5 5 5
fTmTm 1.20 1.18 2.00 2.00 2.00 2.50 2.35 2.55
fS1 2.75 2.00 2.00 2.50 3.50 3.80 5.45 5.40
KB 60.000 122.335 3.363 0.620 0.325 0.1908 0.1670 0.1266
KT 0.1765 0.1879 0.1867 0.1222 0.1065 0.0777 0.0726 0.0557
K1 0.22 0.22 0.22 0.22 0.22 0.22 0.22 0.22
K2 200 200 200 200 200 200 200 200

k1 has units of μM−1s−1; K1 has units of μM−1; equilibrium constants KB, KT and K2, and cooperativity factors fTmTn and fS1 are dimensionless; all other rate constants have units of s−1.