Figure 4. The effect of VOR on Fun-1 staining patterns of C. tropicalis cells within microcolonies.

A, C. tropicalis 2526 (VOR sensitive, MIC 0.0625 µg ml⁻¹) grown for 7 h on PAO (no-drug control). B, Microcolonies of the same strain cultured on 0.0625 µg VOR ml⁻¹ with an increase in Fun-1 staining (particularly for cells on the periphery). C, As panel B, showing the enhancement of Fun-1 staining of peripheral cells by 0.125 µg VOR ml⁻¹. Pictures were taken with identical exposure times (45 ms). Scale bar indicates 20 µm for all images.