Table 2. Effect of staining procedure on calculation of microcolony area and MIC determination using C. albicans 4208.

		PAO method with specified dye	E-test
	Fun-1	Calcofluor	Fun+Calcofluor	Syto9
Average Microcolony Area 1	113	126	131	143
Variance 2	0.048	0.029	0.024	0.022
MIC (AMB) 3	1	0.5	1	1	0.5
MIC (ANI) 3	0.128	0.256	0.128	<0.128	0.064
MIC (CAS) 3	0.25	0.25	<0.128	<0.128	<0.01
MIC (ITR) 3	0.128	0.063	<0.063	<0.063	0.023
MIC (VOR) 3	<0.128	0.128	0.128	<0.128	0.25

¹area in µm².

²calculated from log₁₀ microcolony area.

³MIC in µg ml⁻¹.