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. 2012 Mar 16;7(3):e33838. doi: 10.1371/journal.pone.0033838

Figure 4. GsAPK protein targets to the plasma membrane of N.benthamiana plant cells.

Figure 4

a. Schematic representation of construct used for subcellular localization of the GsAPK protein. b. Subcellular localization assay of the GsAPK:GFP fusion protein in N. benthamiana epidermal cells. Micrographs showing cells expressing GFP (control, upper lane) or GsAPK:GFP (bottom lane) fusion protein, which were examined under bright-field illumination (left), and under fluorescent-field illumination (middle) to examine GFP fluorescence, and by confocal microscopy (right) for an overlay of bright and fluorescent illumination. Scale bar, 50 µm. Arrow, plasma membrane; asterisk, cytosol; triangle, nucleus.