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. 2012 Mar 16;7(3):e33838. doi: 10.1371/journal.pone.0033838

Figure 6. Phenotypic analysis of transgenic Arabidopsis plants under salinity stress and ABA treatments.

Figure 6

a. Response of seed germination to ABA and NaCl in transgenic and wild-type Arabidopsis plants. Seeds from three transgenic and wild-type Arabidopsis were stratified on 0.5× MS agar plates in the absence or presence of 100 mM NaCl or 0.8 µM ABA, respectively. Data are means (±S.E.) of five replicates (each with 100 seeds for each line). b. Phenotypes of wild-type and three transgenic seedlings grown in control or stress condition. 7-day-old WT and transgenic seedlings grown on 0.5× MS were transferred to new solid agar plates supplemented with 125 mM NaCl or 50 µM ABA, respectively. Photographs were taken after 12 days growth. c. Measurements of root lengths of plants shown in b. d. Time course total chlorophyll content of wild-type and three transgenic seedlings under NaCl stress. Three-week-old soil-grown plants were irrigated with 200 mM NaCl for 2 weeks and the total chlorophyll content among the wild-type and three transgenic plants were measured. All values in c and d are means (±S.E.) from three independent experiments (30 seedlings per experiment). **: P<0.01 by Student's t test.