Figure 2. Growth curves of bacterial cultures grown at 39°C.

Wild type and ΔdegP cells, with or without the suppressor mutation, were grown overnight at 30°C. Next day, overnight cultures were diluted to 1∶100 in flasks containing fresh, pre-warmed, Luria broth and growth was resumed at 30°C and 39°C. OD₆₀₀ was measured from bacterial samples withdrawn every 30 minutes. Only 39°C growth curves, obtained from two independent experiments, are shown. All strains grew almost identically at 30°C.