Figure 3. The suppressor mutation reverses the vesiculation phenotype of a ΔdegP strain.

Cultures were grown at 39°C in Luria broth for five hours, after which membranes and vesicles were prepared as described in the Experimental Procedure section. Samples were analyzed by SDS-PAGE and proteins were visualized after Coomassie blue staining. Abbreviations: mem, membrane; ves, vesicle; sup ⁺, the unknown suppressor mutation.