Figure 1.

Cortactin and Arp2/3 are localized in early endosomes and their localization is enhanced by treatment with dynasore. HeLa cells were allowed to internalize Alexa555-trnasferrin (red) for 15 min and treated with 0.5% DMSO (upper panels) or 400 μM dynasore (lower panels) 5 min after internalization. They were stained with anti-cortactin, anti-Arp2/3, or exogenously expressed myc-N-wasp (green). The colocalization of cortactin and Arp2/3, but not of N-wasp, with internalized transferrin was significantly enhanced by dynasore.