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. 2012 Mar 16;7(3):e33322. doi: 10.1371/journal.pone.0033322

Figure 4. Analysis of NLG of IGF1Rβ and IRβ in figitumumab-sensitive cell lines.

Figure 4

A) Immunoblot analysis of different IGF1Rβ migration pattern on SDS-PAGE. Electrophoretic mobility patterns of IGF1Rβ were analyzed in parallel by Western bloting. Experiments were repeated at least three times with similar results. B) Analysis of N-glycosylated IRβ and IGF1Rβ in sensitive cell lines by enzymic deglycosylation with PNGage F. All samples were incubated at 37°C for 12 h with PNGage F. IGF1Rβ and IRβ proteins were analyzed in parallel by Western blotting. The blots shown are representatives of three independent experiments.