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. 2012 Mar 16;7(3):e33596. doi: 10.1371/journal.pone.0033596

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Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) AQP4 IHC signals in swollen end-feet of astrocytes around cerebral vessels in ipsilateral penumbral areas (interaural 1.98 mm). Note the intense AQP4 staining in vehicle-treated vessels after MCAO (arrows, A), which was decreased in both LBP groups (B&C). (D–F) GFAP IHC using adjacent section to AQP4 IHC. Note the GFAP immunoreactivity located around the same cerebral vessels as in the AQP4 immunoreactivity (arrows). Scale bar = 25 µm. (G) Semi-quantification of immunoreactivity of AQP4. *P<0.05, Kruskal-Wallis followed by Dunn's multiple comparison test, n = 5 each group.