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. 2012 Mar 16;7(3):e33610. doi: 10.1371/journal.pone.0033610

Figure 2. Diagnostic PCR of ITS-1 for Culicoides species.

Figure 2

Agarose gels showing PCR samples of 10 individual midges morphologically identified as C. schultzei complex (B1–5, N1–5) and 2 individuals of C. imicola (B6, N6). Same DNA samples were used for phylogeny analysis (Figures 3,4). (A) Genus-specific PCR amplification (B) Species-specific PCR for identification of C. oxystoma (C) Species-specific PCR for identification of the other genetic cluster of C. schultzei complex. NC - negative control, containing no template; M - 100 bp DNA ladder.