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. 2012 Mar 16;7(3):e33627. doi: 10.1371/journal.pone.0033627

Figure 5. Methylation of 15 CpG motifs in the predicted promoter region of the TLR4 gene.

Figure 5

(A) The distribution of the 15 CpG dinucleotides from −2443 to −1361 in the upstream region of the TLR4 gene relative to the translation start site (+1). (B) Genomic DNA from peripheral blood leukocytes of uninfected chickens at 0 h (⁃), susceptible (□) and resistant (⧫) chickens at 16 h TPI was modified with sodium bisulfite, amplified by PCR, cloned, and 12–16 independent clones were sequenced. The frequency of methylated CpGs in each CpG site (data are means of 12 birds for uninfected chicken and 6 birds for susceptible and resistant chickens, respectively) are shown and comparisons were made between susceptible and resistant chickens. The average of % methylation at each CpG site within all 15 CpGs in peripheral blood leukocytes of uncharged chickens (0 h, filled grey bars), susceptible (S, open bars) and resistant (R, filled black bars) chickens at 16 h after infection with S. enteritidis are presented. The vertical bar is the SD from the error mean square of the ANOVA, * indicates P<0.05, ** indicates P<0.01.