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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Aging Cell. 2012 Feb 15;11(2):350–359. doi: 10.1111/j.1474-9726.2012.00794.x

Figure 3. ETS expression in vitro and in vivo.

Figure 3

(A) Immunoblot and data analysis for ETS1 and ETS2 in F344 rat heart from each relative mortality group (4, 8, 30 and 80%). (B) ETS2 immunostaining of heart tissues from F/N rats at a) 2, b) 16, c) 24 and d) 32 months of age. ETS2 (Brown) and TUNEL (Violet) co-staining of hearts from 16 month old animals are shown in e–g, with magnifications shown in f and h. Dark brown staining in e illustrates high levels of ETS2 shown in selected regions of myocardium. Arrows indicate TUNEL positive CMs (f). g and h show TUNEL positive non-CMs lacking strong ETS2 staining. (C) Immunoblot and data analysis for ETS1 and ETS2 protein abundance in rFB, NNCM, and adult left ventricular cardiomyocytes (AdCM), p<0.05. (D) Immunoblot and data analysis for ETS2 protein abundance in vehicle-treated NNCM (Ctls) and NNCMs treated for 24 hours with 5μM of ISO, PE and AngII, * p<0.01.