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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Dev Cell. 2012 Feb 23;22(3):669–677. doi: 10.1016/j.devcel.2011.12.001

Figure 1.

Figure 1

ZNF570 is required for terminal differentiation. (A) Differentiation gene induction heatmap on day 3 of calcium treated keratinocytes (+Ca2+) to induce differentiation as compared to undifferentiated sub confluent control keratinocytes (-Ca2+). (B) ZNF750 mRNA induction during calcium-induced differentiation in vitro [ordinate is log10 scale]. Data show standard error of the mean (SEM). (C) ZNF750 protein induction during calcium-induced differentiation in vitro. (D) Effects of siRNA-mediated ZNF750 depletion (ZNF750i) versus scrambled control (CTL) on terminal differentiation proteins in regenerated organotypic human epidermal tissue; loricrin [LOR; green] and filaggrin [FLG; red], epidermal basement membrane is noted with dashed lines (scale bar=25μm). (E) Terminal differentiation gene mRNA quantitation in epidermal tissue with two independent ZNF750 siRNAs vs. scrambled control. Data show standard error of the mean (SEM). N=3. See also Figure S1.