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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Dev Cell. 2012 Feb 23;22(3):669–677. doi: 10.1016/j.devcel.2011.12.001

Figure 2.

Figure 2

An evolutionarily conserved amino terminal C2H2 zinc finger motif is required for ZNF750-driven terminal differentiation. (A) Induction of terminal differentiation genes in undifferentiated sub confluent cells requires an intact ZNF750 amino terminus. Fold mRNA induction quantified over undifferentiated control cells receiving vector control (CTL). Data shown SEM, n=3. (B) A C2H2 sequence in the evolutionarily conserved ZNF750 amino-terminus. (C) C2H2 sequence requirement in ZNF750-driven differentiation. Differentiation gene expression is shown with expression of full-length ZNF750 with point mutation in the amino-terminal C2H2 motif of either both cysteines (C2 Mut), both histidines (H2 Mut), all four residues (C2H2 Mut) or wild-type full-length control in undifferentiated human keratinocytes. Data shown SEM, n=3. See also Figure S2.