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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Cancer Res. 2012 Jan 26;72(6):1579–1587. doi: 10.1158/0008-5472.CAN-11-2055

Figure 5.

Figure 5

BMK1 depletion promotes tumor metastasis in vivo

(A) 4T1 cells were infected with recombinant lentiviruses encoding shRNA against BMK1 (shBMK1) or with control virus (shCont). Expression levels of epithelial marker, E-cadherin, as well as mesenchymal marker, Vimentin, were examined by immunoblotting in shCont and shBMK1 4T1 cells. BMK1 expression was detected with anti-BMK1 antibody. Protein expression level in shCont cells was set at 1.0. (B) The weight of primary tumors formed by Mock, shCont and shBMK1 cells 3 week post-transplantation. (C) Lungs from mice injected with shBMK1, shCont 4T1, or parental 4T1 (Mock) cells were isolated and stained with H&E to determine cancer metastasis. (D) The number of lung surface metastases formed by Mock, shCont and shBMK1 cells 3 week post-transplantation. Error bars represent ±SEM. (E) Expression levels of epithelial marker, E-cadherin, as well as mesenchymal marker, Vimentin, were examined by immunoblotting in shCont and shBMK1 4T1 lung tumor tissues.