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. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: ACS Chem Biol. 2012 Jan 26;7(3):518–525. doi: 10.1021/cb2004725

Figure 6.

Figure 6

A surface decorated with the αvβ3 integrin-binding small molecule 3 supports the adhesion of differentiated cells but not undifferentiated human embryonic stem (hES) cells. a–b) Flow cytometry analysis of αvβ3 integrin levels in the cell population. a) Human ES cells (WA07) were analyzed by flow cytometry for αvβ3 integrin. b) Differentiated cells derived from embryoid bodies formed from the WA07 hES cell line were analyzed by flow cytometry for αvβ3 integrin. An increase in αvβ3 integrin is seen for the differentiated cell population. c–d) A mixed population of undifferentiated and differentiated cells was seeded onto vitronectin-coated surfaces and immunostained for Oct-4 (green) and counterstained with phalloidin (red) and DAPI (blue). Scale bars, 100 μm. e) High-content imaging was used to determine the number of Oct-4 positive cells from the mixed population bound to the indicated surfaces.