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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Jan 26;32(4):1045–1053. doi: 10.1161/ATVBAHA.111.244210

Figure 2. Frequency analysis of CD31brightCD34+CD45−AC133− cells.

Figure 2

Two strategies for frequency analysis of CD31brightCD34+CD45−AC133− cells from peripheral blood stained with the six-antibody/viability marker panel are shown. In the first strategy (a–d), conventional flow cytometry techniques were utilized. In the second strategy (e–i), PFC techniques were utilized, including the removal of monocytes, red blood cells and dead cells that lead to a more uniform population with a tighter expression profile.