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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Jan 26;32(4):1045–1053. doi: 10.1161/ATVBAHA.111.244210

Figure 5. Prospective identification and isolation of circulating ECFCs by PFC and Immunomagnetic Selection.

Figure 5

Representative PFC analysis of (a) adult peripheral blood mononuclear cells and (b) cord blood mononuclear cells. Putative CD34+CD45− ECFCs are gated in green. (c–e) Cells (b gate) were analyzed for expression of (c) CD31, (d) CD146, and (e) CD105. (f–g) PFC analysis of cord blood mononuclear cells (f) prior to and (g) following isolation of CD146+CD45− cells via MACS. (h) Representative photomicrograph of an ECFC colony derived from the CD146+CD45− fraction of cord blood mononuclear cells 6 days after culture in endothelial-specific media. Scale bar represents 200µm. (i) Low (×1,900) and (j) high (×13,000) magnification electron microscopy of sorted cord blood LIVE/DEAD®−CD14−glyACD31brightCD34+CD45− AC133− cells, is consistent with endothelial morphology.