Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Hypertension. 2012 Feb 21;59(4):763–768. doi: 10.1161/HYPERTENSIONAHA.111.188342

Fig. 1.

Fig. 1

Cross-immunization of WT and ACE KO mice. WT mice were immunized with peritoneal macrophages from either ACE WT or ACE KO mice. After 10 days, the recipient mice were sacrificed and splenocytes were expanded in vitro by restimulating with macrophages equivalent to those used for immunization. After 7 days, lymphocytes were again restimulated with macrophages equivalent to those used for immunization, but for 5 hrs. FACS analysis was then used to stain for CD8 and IFN-γ. The percentage of CD8+ cells that are IFNγhigh is indicated.