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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Heart Rhythm. 2011 Dec 2;9(5):812–820. doi: 10.1016/j.hrthm.2011.11.055

Figure 3. Reduced T-tubule abundance of Cav1.2 in failing human myocardium.

Figure 3

(A) Western blot of protein lysates prepared from human myocardium indicates no significant change of Cav1.2 protein expression level in failing myocardium. (B) A sucrose gradient was generated for isolation of T-tubule membranes from the microsomes prepared from human myocardium. As indicated, fractions recovered from 32%/35% interface are enriched with T-tubule localized proteins like Cav1.2 and NCX1. In the same T-tubule fraction prepared from failing myocardium, Cav1.2 expression is significantly reduced whereas NCX1 expression is preserved. T-tubule Cav1.2 protein level is normalized to NCX1 and presented in the right panel. (n=3 hearts, P<0.05)