Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Pancreas. 2012 Apr;41(3):397–408. doi: 10.1097/MPA.0b013e318236f022

Fig. 2 — A. Two-DE patterns of whole-cell proteins obtained from MIA PaCa-2-2 cells treated with CP-320626 (0 μM, 25 μM, 50 μM, and 100 μM). These 2D gels were stained by SYPRO Ruby. Based on PDQuest software analysis, ratios of normalized spot intensities of four different concentrations of CP-320626 were calculated, and spots showing more than 2.0-fold difference with statistical significance (p<0.05) were selected. A total of 22 spots on 2-D gel were labeled with arrows. B. The validation of three differential proteins (profilin, calreticulin, and GAPDH) by western blotting. Results were consistent with the 2-DE patterns for these three proteins.

Fig. 2 — A. Two-DE patterns of whole-cell proteins obtained from MIA PaCa-2-2 cells treated with CP-320626 (0 μM, 25 μM, 50 μM, and 100 μM). These 2D gels were stained by SYPRO Ruby. Based on PDQuest software analysis, ratios of normalized spot intensities of four different concentrations of CP-320626 were calculated, and spots showing more than 2.0-fold difference with statistical significance (p<0.05) were selected. A total of 22 spots on 2-D gel were labeled with arrows. B. The validation of three differential proteins (profilin, calreticulin, and GAPDH) by western blotting. Results were consistent with the 2-DE patterns for these three proteins.