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. 2012 Jan;4(1):38–51. doi: 10.1002/emmm.201100186

Figure 2. Evi overexpressing ESCs showed increased Wnt response.

Figure 2

  1. Immunofluorescence of endogeneous expression of Evi-YFP in ESC colonies.
  2. Relative mRNA expression levels of Evi were analysed by quantitative RT-PCR. Evi-ESCs expressed increased Evi levels.
  3. Western blot of Evi overexpressing ESCs. Evi-ESCs expressed Evi-YFP fusion protein.
  4. Evi-ESCs stably transfected with 7TCF Firefly luciferase reporter showed increased Wnt reporter activity (*p < 0.05).
  5. Transfection of 7TCF Firefly luciferase reporter containing HEK293 cells with Evi-GFP plasmid led to increased Wnt reporter activity compared to transfection with GFP control vector (**p < 0.01).
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