Figure 7. Downregulation of Evi repressed IL transcription by activation of STAT3.

1. Western blot of U87MG cell lysates after RNAi transfection against Evi (Evi #1 and Evi #3) showed increased levels of phosphorylated STAT3 compared to control transfection. β-Actin was detected as loading control. Representative example of three-independent experiments is shown.
2. Model of the Evi-Wnt-STAT3-IL8 signalling link in human glioblastoma cells. Evi mediated Wnt secretion controls phosphorylation of STAT3. Downregulation of Evi leads to activation of STAT3 by phosphorylation. Phosphorylated STAT3 binds to IL8 promotor and represses IL8 transcription. Downregulation of IL8 reduces glioblastoma cell proliferation and invasiveness.