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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunother. 2012 Apr;35(3):293–298. doi: 10.1097/CJI.0b013e31824300a2

Figure 1.

Figure 1

CMV specific CTL from stem cell donors. Adherent cells from donor peripheral blood mononuclear cells were pulsed with pooled CMV pp65 (donor 1 and 2); pp65 and IE1 peptides (donor 3, 4, 5, 6, and 7) and were incubated with donor peripheral blood lymphocytes. Specific cytotoxicity was measured at day 10 of culture using B cell blasts (BB) infected with either vaccinia encoding pp65 (A) or IE1 (B). IFN-γ producing T cells specific for CMV were analyzed by intracellular staining for pp65 and IE1 (panels C and D). Autologous and allogeneic BB were used as negative controls. All CTL showed < 10% cytotoxicity and < 0.1% IFN-γ production to autologous and allogeneic BB. Note: donor 1 and 2 were not stimulated with IE1.