Table 2.
Properties of the purple form of nitrous oxide reductase (N2OR) isolated from different organisms.
| Paracoccus denitrificans | Paracoccus pantotrophus | Pseudomonas stutzeri | Achromobacter xylosoxidans | |
|---|---|---|---|---|
| anaerobic purification | yes | yes | yes | yes |
| visible spectrum | ||||
| ε550 nma (M−1 cm−1) (dimer) | 10 600 | 8000 | 14 000 | 10 500 |
| A550 nm/A640 nm | 1.7 | 1.9 | 2.1 | 1.8 |
| EPR | ||||
| g‖ | 2.224 | 2.181 | 2.180 | 2.180 |
| g⊥ | 2.042 | 2.038 | 2.030 | 2.019 |
| A (gauss) | 35 | 38 | 38 | 40 |
| specific activity (U mg−1)b | 122 | 3 | 4 | 6 |
| reference | [22] | [15] | [21] | [23] |
aValue determined based on the total protein content for the fully oxidized purple N2OR.
bThe specific activity was determined using benzyl viologen (Pseudomonas stutzeri and Paracoccus denitrificans) or methyl viologen (Paracoccus pantotrophus and Achromobacter xylosoxidans) as electron donor.