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. 2012 May 5;367(1593):1204–1212. doi: 10.1098/rstb.2011.0311

Table 4.

Specific activity of Marinobacter nitrous oxide reductase (N2OR) isolated from the different growth conditions. The table also shows the main redox state of the CuZ centre in each redox state. asc, ascorbate; dth, dithionite.

redox state ‘aerobic’
‘anaerobic1’
‘anaerobic2’
activity (U mg−1) redox state CuZ centrea activity (U mg−1) redox state CuZ centrea activity (U mg−1) redox state CuZ centrea
fully oxidized 0.4 60% [2Cu2+ : 2Cu+] 0.4 60% [2Cu2+ : 2Cu+] 1.1 [1Cu2+ : 3Cu+]
asc-reduced 0.3 60% [2Cu2+ : 2Cu+] 0.3 60% [2Cu2+ : 2Cu+] 0.9 [1Cu2+ : 3Cu+]
dth-reduced 0.2 [1Cu2+ : 3Cu+] 0.3 [1Cu2+ : 3Cu+] 0.2 [1Cu2+ : 3Cu+]
fully reduced 88.0 [4Cu+] 96.1 [4Cu+] 92.4 [4Cu+]

aThe CuZ centre content for ‘aerobic’ and ‘anaerobic2’ N2OR samples was determined based on the EPR spectra, whereas the one indicated for ‘anaerobic1’ was estimated by comparing its visible spectral features with those of the ‘aerobic’ sample.