Table 4.
redox state | ‘aerobic’ |
‘anaerobic1’ |
‘anaerobic2’ |
|||
---|---|---|---|---|---|---|
activity (U mg−1) | redox state CuZ centrea | activity (U mg−1) | redox state CuZ centrea | activity (U mg−1) | redox state CuZ centrea | |
fully oxidized | 0.4 | 60% [2Cu2+ : 2Cu+] | 0.4 | 60% [2Cu2+ : 2Cu+] | 1.1 | [1Cu2+ : 3Cu+] |
asc-reduced | 0.3 | 60% [2Cu2+ : 2Cu+] | 0.3 | 60% [2Cu2+ : 2Cu+] | 0.9 | [1Cu2+ : 3Cu+] |
dth-reduced | 0.2 | [1Cu2+ : 3Cu+] | 0.3 | [1Cu2+ : 3Cu+] | 0.2 | [1Cu2+ : 3Cu+] |
fully reduced | 88.0 | [4Cu+] | 96.1 | [4Cu+] | 92.4 | [4Cu+] |
aThe CuZ centre content for ‘aerobic’ and ‘anaerobic2’ N2OR samples was determined based on the EPR spectra, whereas the one indicated for ‘anaerobic1’ was estimated by comparing its visible spectral features with those of the ‘aerobic’ sample.