Figure 2.

The typical denitrification phenotype of P. denitrificans in response to oxygen depletion in a 50 ml batch of Sistrom's medium with 2 mM NO₃⁻ and pH = 7. The graph shows O₂ and NO concentration in the liquid (as µM and nM, respectively) and cumulated N₂ production (as µmol flask⁻¹) . NO concentrations are sustained around 15 nM (i.e. 20 nmol flask⁻¹) during the period of active denitrification (which lasts until all the nitrate has been depleted and recovered as N₂). The transient accumulation of N₂O is below the detection limit of the system which is approximately 2 nmol flask⁻¹ . Selected data from Bergaust et al. [37].