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. 2012 Feb 21;109(11):E665–E672. doi: 10.1073/pnas.1110215109

Fig. 2.

Fig. 2.

The MtBcp1 promoter drives expression before and during arbuscule formation and confirms the importance of gene expression coordinated with arbuscule development for localization in the periarbuscular membrane. (A) In cells with intracellular hyphae (IH) but without arbuscule branches (cell 1), pMtBcp1:MtPT4-GFP localizes in the ER as indicated by the peripheral and perinuclear ER pattern. Upon arbuscule branching (cells 2 and 3), MtPT4 accumulates to high levels in the periarbuscular membrane (arrowhead). (B) pMtBcp1:MtPT1-GFP localizes in the plasma membrane of noncolonized cortical (NC) cells, in cells with intracellular hyphae, and in the periarbuscular membrane of cells with branched arbuscules. (C) Coexpression of pMtBcp1:MtPT1-GFP and pMtBcp1:AtPIP2a-mCherry in two cells with young arbuscules just beginning to branch illustrates plasma membrane localization of both proteins but contrasting perinuclear ER localization of AtPIP2a (N) and periarbuscular membrane localization of MtPT1 (arrowhead). (D) Coexpression of two AtPIP2a fusions reveals plasma membrane and ER localization for pMtBcp1:AtPIP2a-mCherry (red) but only ER localization for pMtPT4:AtPIP2a-GFP (green). (Scale bars: 20 μm.)