Fig. 3.
SHP2 promotes GH-evoked ERK1/2 activation, but does not influence the JAK2/STAT5 or PI3K/Akt pathways. (A and B) 3T3F442A ΔSHP2ind cells were treated with doxycycline (+dox) or left untreated (−dox), serum-starved, and then stimulated with GH during the indicated times, lysed and processed for Western blot analysis. (C and D) HEKGHR-GFP or HEKGHR-shRNA cells were processed as in A. (E and F) 3T3F442A cells were infected with adenoviruses encoding GFP, His-tagged SHP2-WT or SHP2-C459G, and then processed as in A. (B, D, and F) Quantifications of P-ERK1/2 Western blots from experiments described in A, C, and E.