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. 2012 Feb 27;109(11):4257–4262. doi: 10.1073/pnas.1119803109

Fig. 5.

Fig. 5.

NS-associated SHP2 mutants enhance GH-induced ERK1/2 activation. (A and B) HEKGHR cells were cotransfected with ERK1-His and His-tagged SHP2 WT, D61del, or N308D variants, or the empty vector (−), and then processed as in Fig. 4E. (C and D) 3T3F442A cells were infected with adenoviruses encoding GFP, SHP2-N308D, or SHP2-D61del, serum starved, then GH stimulated for 10 min and processed for indicated Western blot analysis. (E and F) Untreated (−dox) or 0.005 μg/mL doxycycline-treated (+dox) 3T3F442A ΔSHP2/N308D and 3T3F442A ΔSHP2/D61del cells were GH stimulated for 10 min, lysed, and processed for indicated Western blot analysis. (G and H) Fasted WT and NS mice were injected i.p. with 8 μg/g GH (+) or PBS (−) for 10 min, and then euthanized. Equal amounts of protein from liver extracts were processed for Western blot analysis. (B, D, F, and H) Quantification of immunoblots described in A, C, E, and G. Only significant differences vs. control are indicated.