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Synthesis of the AuNP-ligand conjugate. (A) Synthesis of thiol analogs of NSC333751 and a control for linking to AuNP. (B) Comparison of binding of the thiol analogue 2 (red spectrum) and NSC333751 (green spectrum) to SUMO-3, both at a ligand:protein ratio of approximately 3∶1. Both spectra are superimposed with that of free SUMO-3 (black). (C) Schematic diagram of the synthesis of the ligand-AuNP conjugates 4 and 5.
