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ATM8 kinase activity is temperature-sensitive. Shown is Western blot analysis of H2Av-pS137 in adult head extracts from flies of the indicated genotypes exposed (+) or not exposed (−) to IR. Analyses of WT, ATM8/+, and ATM8 flies at 18 °C (lanes 1–6) and 25 °C (lanes 7–12) are shown. The Top panels show a short exposure of the Middle panels. The Bottom panels were probed for α-tubulin, which served as a loading control.
