Fig. 1.
ABCB8 KO in the heart. (A) Southern blot analysis of WT (lane 1) and targeted ES cells (lane 2). Homologous recombination results in a detection of a smaller band after genomic DNA is digested with Sca I restriction enzyme, as shown in A. (B) PCR results of the 5′ arm of ABCB8 heterozygote and homozygote floxed mice. Presence of LoxP sequence results in a larger fragment. (C) Tamoxifen-mediated genetic ablation of ABCB8 in the heart. DNA samples from two untreated (labeled 1 and 2) and two tamoxifen-treated (labeled 3 and 4) mice are shown. (D) ABCB8 mRNA levels are significantly lower in MCM × ABCB8f/f mice 2 wk after treatment with per-oral (PO) tamoxifen compared with WT ABCB8+/+ mice. (E) Levels of ABCB8 protein after treatment of MCM × ABCB8f/f mice with per-oral tamoxifen for 2 wk are significantly lower than in control mice. (F) Summary of densitometry analysis of Western blot (n = 3). Data are presented as mean ± SEM (*P < 0.05).