. Author manuscript; available in PMC: 2013 Feb 28.

Published in final edited form as: J Am Chem Soc. 2012 Feb 15;134(8):3912–3916. doi: 10.1021/ja212182q

Table 2.

Selenoproline Ligation: Substrate Scope.^a


Entry	C-terminal Peptide	Product	Time / Yield
1		H-ALLVNSSGPWEPLQ-OH 47	4.5 h, 84%
2		H-ALLVNSSAPWEPLQ-OH 49	4.5 h, 88%
3		H-ALLVNSSFPWEPLQ-OH 51	10 h, 80%
4		H-ALLVNSSVPWEPLQ-OH 53	12 h, 66%
5		H-ALLVNSSPPWEPLQ-OH 55	24 h, NR

Key: (a) MPAA, Ligation Buffer (6 M Gn•HCl, 200 mM NaH₂PO₄), pH 7.4–7.6, 23 °C; (b) DTT, Ligation Buffer; then TCEP, pH 5.0–6.0, 23 °C. P1: ALLVNSS−; P2: −WEPLQ.