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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: J Neurosci Res. 2009 Jun;87(8):1848–1857. doi: 10.1002/jnr.21996

Figure 4. Proliferating cell survival and neuronal differentiation.

Figure 4

BrdU was administered beginning two weeks postinjury for seven consecutive days. Animals were euthanized three weeks after the final injection (six weeks postinjury) and assessed for proliferating cell survival (BrdU+, red) and neuronal differentiation (double-labelling with NeuN, green) within the dentate gyrus. The majority of BrdU+ cells were located in the subgranular zone (A, GPx Tg sham) where double-labeled cells could also be found (B, arrows). As with Ki-67, the interaction between genotype and injury had no effect on proliferating cell survival (p=0.56) but injury resulted in a significant reduction (p=0.016, C). A similar pattern was seen with neuronal differentiation, with injury trending towards an effect (p=0.087, D). Thus, while injury reduces proliferating cell survival and possibly neuronal differentiation, GPx overexpression is unable to rescue those changes. No differences were seen in the percentage of BrdU+ cells that differentiated into neurons (E). Scale bars: A 100μm; B 50μm.