Skip to main content
. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: Cytometry A. 2011 Feb 9;79(3):227–232. doi: 10.1002/cyto.a.21029

Figure 1.

Figure 1

Workflow of the automated wound healing analysis (clockwise from top left). First, cells are seeded and allowed to proliferate to confluence in a multi-well tissue culture plate. Then LEAP™ is used to create wounds in each well by laser ablation. The plate is then washed to remove cell debris, and if necessary the wells are dosed with a drug or compound. The LEAP™ is then used to image every well in bright field mode every 24 h. Image analysis is performed by our texture segmentation algorithm to determine the wound area for each well and time point. The wound healing is then calculated and analyzed to determine the effect of a given drug or compound.