Figure 2.

Dose-response effects of test drugs on the release of [³H]MPP⁺ and [³H]5-HT from rat brain synaptosomes in vitro, under conditions optimized for NET (a), DAT (b), and SERT (c). Dose-response curves were constructed by incubating various concentrations of each test drug with synaptosomes that had been preloaded with tritiated substrate, either [³H]MPP⁺ or [³H]5-HT. Test drugs were mephedrone (4MMC), methylone (MDMC), 3,4-methylenedioxymethamphetamine (MDMA), and methamphetamine (MA). Data are mean±SD for N=6 (for NET assays) or N=3 (DAT and SERT assays) separate experiments.